Bacillus cereus Selective Agar (PEMBA) Detail
This is a medium for the selective isolation and enumeration of Bacillus cereus in food samples. It is said to be particularly suitable for the detection of small numbers of Bacillus cereus particularly in the presence of other contaminating organisms. The medium is made selective by the inclusion of Polymixin and Sodium Pyruvate is also present which is said to improve Egg Yolk precipitation and enhance sporulation. As Bacillus cereus is Mannitol Negative the colonies are bluish in colour, due to the presence of the Bromothymol Blue Indicator, with a surrounding precipitate of the same colour due to Lecithinase production (from the Egg Yolk).
Bacillus cereus Selective Agar (PEMBA) P0032
Product Specification
- Categories: Food, Water & Environmental
- Pack Size: 10 x 90 mm plates
- Shelf Life: 12 weeks
- Storage Temp: 2-8 °C
- Appearance: Firm Gel
- Colour: Lime Green
- Recommended Incubation: 30 ± 1°C for 44 ± 4 hours
- pH: 7.2 ± 0.2
- Fill Volume: 18.0 ± 2.0ml
Quality Control
| Organisms | Ref. No | Result |
|---|---|---|
| Bacillus cereus | ATCC® 10231 | Blue colonies with precipitation halo |
| Bacillus subtilis | ATCC® 6633 | White colonies with no precipitation halo |