DNase Agar (DNA) (P0030) Detail
Medium used primarily in clinical laboratories to differentiate Staphylococcus aureus from other Staphylococci based on deoxyribonuclease activity. Following incubation of the plate and confirmation of a pure growth of Staphylococci, the surface of the medium is covered with a small quantity of 1M Hydrochloric Acid to precipitate the DNA. Staphylococcus aureus produce DNase enzymes that hydrolyse DNA resulting in a clear area around the colonies, described as being DNase positive, whereas coagulase negative Staphylococci do not produce clearing.
DNase Agar (DNA) (P0030)
- Category: Clinical & Veterinary / Food, Water & Environmental
- Pack Size: 10 x 90 mm plates
- Shelf Life: 12 weeks
- Storage Temp: 2-8 °C
- Appearance: Firm Gel
- Colour: Straw
- Recommended Incubation: 30 ± 1°C for 24 ± 2 hours
- pH: 7.3 ± 0.2
- Fill Volume: 18.0 ± 2.0ml
Quality Control
Organisms | Ref. No | Result |
---|---|---|
Staphylococcus aureus | ATCC® 25923 | Good growth, clear zones |
Staphylococcus epidermidis | ATCC® 12228 | Good growth; no zones |