DNase Agar (DNA) (P0030) Detail

Medium used primarily in clinical laboratories to differentiate Staphylococcus aureus from other Staphylococci based on deoxyribonuclease activity. Following incubation of the plate and confirmation of a pure growth of Staphylococci, the surface of the medium is covered with a small quantity of 1M Hydrochloric Acid to precipitate the DNA. Staphylococcus aureus produce DNase enzymes that hydrolyse DNA resulting in a clear area around the colonies, described as being DNase positive, whereas coagulase negative Staphylococci do not produce clearing.

DNase Agar (DNA) (P0030)

  • Category: Clinical & Veterinary / Food, Water & Environmental
  • Pack Size: 10 x 90 mm plates
  • Shelf Life: 12 weeks
  • Storage Temp: 2-8 °C
  • Appearance: Firm Gel
  • Colour: Straw
  • Recommended Incubation: 30 ± 1°C for 24 ± 2 hours
  • pH: 7.3 ± 0.2
  • Fill Volume: 18.0 ± 2.0ml

Quality Control

Organisms Ref. No Result
Staphylococcus aureus ATCC® 25923 Good growth, clear zones
Staphylococcus epidermidis ATCC® 12228 Good growth; no zones