BCYEK with GVPC* (BYC-C) P0048
This is a selective medium for the isolation of Legionella spp is used primarily in water and environmental laboratories. The base agar contains Yeast Extract as a source of protein, Charcoal to neutralize growth-inhibiting substances and is supplemented with Ferric Pyrophosphate as a source of iron, L-cysteine hydrochloride, and α-ketoglutarate to form amino acid and chelate respectively. ACES buffer is incorporated to maintain the optimal pH for growth. The medium is made selective by the addition of Glycine, Vancomycin & Polymyxin B to inhibit the majority of gram positive and gram-negative organisms and Cycloheximide is also included to inhibit yeasts and fungi.
BCYEK with GVPC* (BYC-C) P0048
- Category: Food, Water & Environmental
- Pack Size: 10 x 90 mm plates
- Shelf Life: 12 weeks
- Storage Temp: 2-8 °C
- Appearance: Firm Gel
- Colour: Black
- Recommended Incubation: 37°C in high humidity (90% recommended) for up to 10 days. It is also recommended that cultures will benefit from incubation in an atmosphere containing 2.5-5% CO2
- pH: 6.9 ± 0.2
- Fill Volume: 20.0 ± 2.0ml
Quality Control
Organisms | Ref. No | Result |
---|---|---|
Legionella pneumophila | NCTC 11192 | White/grey translucent colonies |
Legionella anisa | NCTC 11974 | White/grey translucent colonies |
Escherichia coli | NCTC 12241 | Inhibited |
Staphylococcus aureus | NCTC 12981 | Inhibited |