Chromogenic Coliform Agar (CCA) P0054

Chromogenic Coliform Agar (CCA) conforms to the ISO 9308-1 guidelines for the detection, enumeration and isolation of coliforms and more specifically Escherichia coli in water samples by the membrane-filtration technique. The colonial differentiation is provided by the chromogenic substrates, Salmon-GAL and X-glucuronide. Salmon-GAL is used for the detection of β-D-galactosidase enzymatic activity. X-glucuronide is used for the detection of β-D-Glucuronidase enzymatic activity. β-D-galactosidase, expressed by all coliforms, cleaves the Salmon-GAL substrate and producing red/pink coloured colonies. Unlike other coliforms, Escherichia coli leaves both Salmon-GAL and X-glucuronide producing a violet/blue coloured colony. Tryptophan is used to increase detection reliability by improving the indole reaction. The peptones, sodium pyruvate and sorbitol support bacterial growth and simple recovery of sub-lethal thermally injured coliforms. Sodium di-hydrogen phosphate and di-sodium hydrogen phosphate phosphate buffer the medium and sodium chloride is used to achieve osmotic balance. The selectivity is attained by the addition of Tergitol® 7 as it inhibits the growth of Gram-positive bacteria.

Chromogenic Coliform Agar (CCA) P0054

• Category: Food, Water & Environmental
• Pack Size: 10 x 90 mm plates
• Shelf Life: 6 weeks
• Storage Temp: 2-8 °C
• Appearance: Firm Gel
• Colour: Straw
• Recommended Incubation: 37 ± 1°C for 21 ± 3 hours
• pH: 6.8 ± 0.2
• Fill Volume: 20.0 ± 2.0ml